中国陆生蓝藻新记录属—科马雷克藻属(Komarekiella)
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摘要：
蓝藻具较强的适应能力，不仅能生活在淡水和海洋等各种水体生境中，也能在土壤、沙漠、极地、温泉等极端生境中生存。通过野外样品采集，从中国广西壮族自治区桂林市九马画山生物土壤结皮中成功分离到1株蓝藻藻株JM-1001，采用形态学、生态学和分子生物学相结合的多相分析方法对其进行了系统发育分析和分类特征研究。该藻株为单细胞、丝状体或群体生活，具有明显的生活史，具异形胞和厚壁孢子，与科马雷克藻属Komar- ekiella形态特征极其相似。基于16SrRNA基因系统发育分析表明，该藻株聚集在科马雷克藻属支系中，并且JM-1001和大西洋科马雷克藻Komarekiella atlantica的序列紧密聚为一个系统进化分支，具有很高的步展值(98/93/1)。藻株JM-1001与模式种大西洋科马雷克藻K.atlantica的16S rRNA基因相似性 ⩾99. 9%。同时，藻株JM-1001和大西洋科马雷克藻 ITS二级结构中的D1-D1'、Box-B 和 V3 螺旋结构一致。这些结果共同支持了本研究分离的藻株属于模式种大西洋科马雷克藻，该属是中国首次发现的新记录属。
Cvanobacteria, with strong adaptability, living in not only freshwater and marine environments, but also desert, polar, hot springs and other extreme habitats, In this study, a strain of cyanobacteria, JM-1001, was isolated and purified from the soil laver of liumabuashan, Guilin City, Guangxi Zhuang Autonomous Region, China. The phvlogenetic and taxonomic characteristics of JM-1001 were studied based on polvphasic analysis combining morphological. ecological and molecular data. Morphological characteristics of the cyanobacteria strain was very similar to Komarekiella, which with unicellular, filamentous or in colonies, distinct life history, heterocytes and akinete. The phylogenetic analysis of 16S rRNA gene sequence manifested that this strain was clustered in the Komarekiella clade, and JM-1001 and Komarekiella atlantica were closely clustered into a phylogenetic clade with high bootstrap values(98/93/1). The 16S rRNA gene similarity between JM-1001 and K. atlantica is 99.9%, which supports it to be a member of K. atlantica. In addition, the 16S-23S ITS secondary structure of JM-1001 is consistent with that of K. atlantica. All results supported it to be a member of K. atlantica. And the genus Komarekiella is a newly recorded genus in China.
作者：
陈嘉欣，席雨，李姝衡，蔡芳芳
Chen Jiaxin， XiYu， LiShuheng，CaiFangfang
机构地区：
武汉轻工大学动物科学与营养工程学院；生态环境部太湖流域东海海域生态环境监督管理局生态环境监测与科学研究中心
引用本文：
陈嘉欣，席雨，李姝衡等。中国陆生蓝藻新记录属—科马雷克藻属(Komarekiella)[J].学报(自然科学版) ,2025,53(2) :73-81. (Chen Jiaxin,Xi Yu,Li Shuheng,et al.Komarekiella(Nostocale,Cyanobacteria):a terrestrial novel recorded genus from China[J] . Journal of Henan Normal University(Natural Science Edi-tion),2025,53(2) :73-81.DOI:10.16366/j.cnki.1000-2367.2023.11.07.0002.)
基金：
国家自然科学基金
关键词：
蓝藻；新记录属；系统发育；分类
cyanobacteria;new recorded genus; phylogenetic; taxonomy
分类号：
X52;Q949.22
