淡绿杜洛埃藻(Drouetiella lurida)---分离自中国西藏的蓝藻新记录种

摘要：

杜洛埃藻属(Drouetiella Mai, Johansen &.Pietrasiak)中的物种具有形态可塑性，容易与细点丝藻科(Oculatellaceae)的其他物种混淆，该属中的藻株从陆地生物群落中分离，采用包括分子手段、形态特征和生态信息相结合的多相方法进行表征。从中国西藏自治区拉萨市的潮湿土壤分离出一株丝状蓝藻,编号为 SXACC0052，形态上与淡绿杜洛埃藻(Drouetiella lurida(Gomont)Mai,Johansen &. Pietrasiak)相似，藻丝都呈现橄榄绿色，有无色且坚固的鞘,横壁不收缩或稍微收缩,没有观察到假分枝。16SrRNA基因与16S-23SrRNA ITS序列显示出这株蓝藻与淡绿杜洛埃藻有较高的相似度，并且在系统发育树中聚为一支，步展值分别为98/78/1和85/99/0.89。此外，这株丝状蓝藻的 ITS二级结构与淡绿杜洛埃藻的结构相似度较高，进一步表明这株丝状蓝藻属于淡绿杜洛埃藻,杜洛埃藻属是我国首次报道的新记录属，淡绿杜洛埃藻是我国首次报道的新记录种，该种的发现丰富了我国蓝藻物种多样性，为保护和持续了解藻种资源提供理论依据。

Species in the genus Drouetiella Mai, johansen & Pietrasiak are morphologically plastic, easily confused with other species in the family of Oculatellaceae, in which strains are isolated from terrestrial communities and characterized using a polyphasic approach that includes molecular, morphological and ecological information. A filamentous cyanobacteria was isolated from the moist soil of lhasa, Xizang Autonomous Region, China, named SXACC0052. The morphology of isolated strain was similar to Drouetiella lurida((Gomont)Mai, Johansen &. Pietrasiak), the trichomes were olive-green, with colorless and firm sheaths, the cross-walls were not constricted or slightly contracted, no false-branching was observed. The comparative analysis of 16S rRNA gene and 16S-23S rRNA ITS sequences showed that the strain shared high similarities with Drouetiella lurida and clustered into a single branch in the phylogenetic tree with bootstrap value of 98/78/1 and 85/99/0.89, respectively. In addition, the ITS secondary structure of this filamentous cyanobacteria has a high similarity to that of Drouetiella lurida, which further indicated that this filamentous cyanobacteria belonged to Drouetiella lurida. Drouetiella is a new record genus reported for the first time in China, Drouetiella lurida is a new record species reported for the first time in China. The discovery of this species enriches the species diversity of cyanobacteria in China and provides a theoretical basis for the conservation and continuous understanding of algae resources.

作者：

王捷，张婷，蔡芳芳，董建新，李艳晖，刘琪

Wang Jie，Zhang Ting， Cai Fangfang，Dong Jianxin， Li Yanhui，Liu Qi

机构地区：

太原师范学院生物科学与技术学院；太原师范学院汾河流域地表过程与资源生态安全山西省重点实验室；武汉轻工大学动物科学与营养工程学院；山西大学生命科学学院

引用本文：

王捷，张婷，蔡芳芳等。淡绿杜洛埃藻(Drouetiella lurida)--分离自中国西藏的蓝藻新记录种 [J] . 学报(自然科学版) , 2025, 53(2) : 63-72. (Wang Jie, Zhang Ting, Cai Fangfang, et al.Drouetiella lurida: a new record species of cyanobacteria isolated from Xizang,China[J] .Journalof Henan Normal University(Natural Science Edition) ,2025,53(2) :63-72. DOI:10. 16366/j. cnki.1000-2367. 2024. 03. 20. 0003.)

基金：

国家自然科学基金；山西省应用基础研究计划（面上项目）；中央水生态环境保护专项项目（拉萨河流域水生态调查评估）

关键词：

淡绿杜洛埃藻；西藏；蓝藻；新记录；系统发育

Drouetiella lurida ; Xizang; cyanobacteria; new record; phylogeny

分类号：

Q949. 22  

淡绿杜洛埃藻(Drouetiella lurida)---分离自中国西藏的蓝藻新记录种.pdf